Application Range and Method of Defoamer Fermentation

Jan 26, 2021

Fermentation defoamer is a kind of defoamer specially designed for fermentation process. It is divided into: oily defoamer, silicone defoamer, and compound oil defoamer. Such as: (Weifang Jinshuiyuan J defoamer, Hangzhou Linhua defoamer, Tieling Jinfeng defoamer, Changsha Shanke defoamer, etc.) used for defoaming in various fermentation production processes, such as erythromycin and linomycin Various fermentation defoaming processes such as avermectin, gentamicin, penicillin, citric acid, lysine, yeast production, etc., are also widely used in veterinary drug processing and later extraction processes.

Foam
1. The intensity of aeration and stirring. Large aeration and strong stirring can increase foam. Therefore, in the early stage of fermentation, the nutrient components of the medium are less consumed, and the medium is rich in ingredients and easy to foam. Small ventilation should be started first, and then gradually increased. The same applies to the stirring speed. Defoamers can also be added to the base material.
2. Medium ratio and raw material composition The medium is rich in nutrients, high in viscosity, and produces many and lasting bubbles, and it is difficult to stir in the early stage.
3. Strains, seed quality and inoculum amount:
The strain is of good quality, grows fast, and the soluble nitrogen source is used quickly, and the probability of foam generation is less. If the strain grows slowly, the inoculum can be increased
4. Sterilization quality:
The sterilization quality of the culture medium is not good, the sugar and nitrogen are destroyed, the growth of microorganisms is inhibited, the seed hyphae is autolyzed, and a large amount of foam is produced. The addition of antifoaming agent is also ineffective.
How to choose a defoamer for fermentation.

Select points
1. Insoluble or insoluble in foaming liquid
To break the foam, the defoamer should be concentrated and concentrated on the foam film. In the case of foam breaker, it should be concentrated and concentrated in an instant, and in the case of foam suppression, it should be kept in this state frequently. Therefore, the defoamer is supersaturated in the foaming liquid, and only insoluble or insoluble can it easily reach the supersaturated state. Insoluble or poorly soluble, it is easy to gather at the gas-liquid interface, and it is easy to concentrate on the bubble film, and it can play a role at a lower concentration. For defoamers used in water systems, the active ingredient molecules must be strong hydrophobic and weakly hydrophilic, with an HLB value in the range of 1.53 for the best effect.
2. The surface tension is lower than the foaming liquid
Only when the defoamer has a small intermolecular force and the surface tension is lower than that of the foaming liquid, the defoamer particles can be immersed and expanded on the foam film. It is worth noting that the surface tension of the foaming liquid is not the surface tension of the solution, but the surface tension of the foaming solution.
3. There is a certain degree of affinity with the foaming liquid
Since the defoaming process is actually a competition between the foam collapse speed and the foam generation speed, the defoamer must be able to quickly disperse in the foaming liquid in order to quickly play a role in a wider range of the foaming liquid. To make the defoamer diffuse faster, the active ingredients of the defoamer must have a certain degree of affinity with the foaming liquid. The active ingredient of the defoamer is too close to the foaming liquid and will dissolve; too thin and difficult to disperse. The effectiveness will be good only if the relationship is suitable.
4. No chemical reaction with foaming liquid
The defoamer reacts with the foaming liquid. On the one hand, the defoamer will lose its effect, and on the other hand, it may produce harmful substances that affect the growth of microorganisms.
5. Low volatility and long action time

Efficacy performance
1. Good foam suppression performance.
2. Low dosage and high efficiency.
3. Fast defoaming speed and good stability.
4. This product is non-toxic and odorless, which is beneficial to production safety.
5. Acid, alkali and high temperature resistance.
6. Effective substance content 98%; PH value (1% aqueous solution) 6-8.

Use:
This product is a high-efficiency organic silicon defoamer specially designed for fermentation technology, used for defoaming in various fermentation production processes, such as erythromycin, lindamycin, abamectin, gentamicin, penicillin , Citric acid, lysine, yeast production and other fermentation and defoaming processes are also widely used in veterinary drug processing and later extraction processes.

Instructions
①This product can be added to the base material at one time
②Continuously drip or flow into the fermentation broth
③Amount used: When used alone, it is 0.15%-0.2% of the ingredient volume. If used with 0.01-0.03% foam enemy, 0.05-0.1% can achieve a good defoaming effect.
1) According to the characteristics of the fermentation process, it can be added to the base material, or it can be added after high temperature sterilization in the later stage.
2) When used, it can be miscible with vegetable oils such as soybean oil for defoaming.
3) The recommended dosage is 0.1‰-0.3‰, which is 1/3-1/2 of the used amount of polyether and silicon defoamers.
4) It has been proved by use that a reasonable addition ratio is determined according to the characteristics of the fermentation process before use, which is easy to increase the yield; excessive addition of antifoaming agent may have a negative impact on the growth of the bacteria.
Product Safety
1. It is recommended to take a small sample of foaming liquid to simulate actual use conditions for defoaming test and determine the best addition amount of defoamer before selection.
2. When used for defoaming in medicine and food, it should strictly follow the requirements of pharmacopoeia and relevant laws and regulations, and it should be disinfected and sterilized before use.